Stain in half of time:

The Original:

For LCM:

Automated Search:



Sperm HY-LITER the Original

Positive Identification of Sperm
Meets New Audit Standards for Evidence Preservation
Microscopic Screening of Sperm
Human Specific – Monoclona Antibody-based

Fluorescent Detection – Debris Does Not Interfere
They glow- scan at 40x

Step 1: Attach Cells to Slide - 10 min incubation
Step 2: Sample Preparation - 30 min incubation
Step 3: Blocking - 30 min incubation
Step 4: Labeling and Staining - 30 min incubation
Step 5: Mounting & Cover Slip (optional) - 20 min

Hands-on Time: less than 10 minutes!
Smear Slides: Included Hydrophobic Barrier Pen Provides a Thin Film to Define Target Area
Protocols - Instructions - Technical Documentation - Included in Every KitSperm HY-LITER abstract :Int J Legal Med DOI 10.1007/s00414-008-0271-1

Requirement: Filter for Dapi; Filetr for Alexa 488


Sperm Hy-Liter™: an effective tool for the detection of spermatozoa in sexual assault exhibits. De Moors A, Georgalis T, Armstrong G, Modler J, Frégeau CJ. Forensic Sci Int Genet. 2013 May;7(3):367-79.

Low copy number DNA profiling from isolated sperm using the aureka®-micromanipulation system. Schneider C, Müller U, Kilper R, Siebertz B. Forensic Sci Int Genet. 2012 Jul;6(4):461-5.

Developmental validation of the SPERM HY-LITER™ kit for the identification of human spermatozoa in forensic samples. Miller KW, Old J, Fischer BR, Schweers B, Stipinaite S, Reich K. J Forensic Sci. 2011 Jul;56(4):853-65

Automatic detection of spermatozoa for laser capture microdissection. Vandewoestyne M, Van Hoofstat D, Van Nieuwerburgh F, Deforce D. Int J Legal Med. 2009 Mar;123(2):169-75.

Immunohistochemical staining of human sperm cells in smears from sexual assault cases. Christoffersen S. Int J Legal Med. 2011 Nov;125(6):887-90.


presentation-spermhyliter Presentation from PPT
manual-spermhyliter Manual 46 Mbytes
validationspermhyliter Validation Study
technical spermhyliter Technical Information
protocaol sperm hyliter Protocol
flyer hyliter Flyer
DTT spermhyliter DTT Protocol